Default Mode Network extraction of ADHD dataset#

This example shows a full step-by-step workflow of fitting a GLM to data extracted from a seed on the Posterior Cingulate Cortex and saving the results.

More specifically:

  1. A sequence of fMRI volumes are loaded.

  2. A design matrix with the Posterior Cingulate Cortex seed is defined.

  3. A GLM is applied to the dataset (effect/covariance, then contrast estimation).

  4. The Default Mode Network is displayed.

Note

If you are using Nilearn with a version older than 0.9.0, then you should either upgrade your version or import maskers from the input_data module instead of the maskers module.

That is, you should manually replace in the following example all occurrences of:

from nilearn.maskers import NiftiMasker

with:

from nilearn.input_data import NiftiMasker
import numpy as np

from nilearn import datasets, plotting
from nilearn.maskers import NiftiSpheresMasker

from nilearn.glm.first_level import FirstLevelModel
from nilearn.glm.first_level import make_first_level_design_matrix

Prepare data and analysis parameters#

Prepare the data.

adhd_dataset = datasets.fetch_adhd(n_subjects=1)

# Prepare timing
t_r = 2.
slice_time_ref = 0.
n_scans = 176

# Prepare seed
pcc_coords = (0, -53, 26)

Estimate contrasts#

Specify the contrasts.

seed_masker = NiftiSpheresMasker([pcc_coords], radius=10, detrend=True,
                                 standardize=True, low_pass=0.1,
                                 high_pass=0.01, t_r=2.,
                                 memory='nilearn_cache',
                                 memory_level=1, verbose=0)
seed_time_series = seed_masker.fit_transform(adhd_dataset.func[0])
frametimes = np.linspace(0, (n_scans - 1) * t_r, n_scans)
design_matrix = make_first_level_design_matrix(frametimes, hrf_model='spm',
                                               add_regs=seed_time_series,
                                               add_reg_names=["pcc_seed"])
dmn_contrast = np.array([1] + [0] * (design_matrix.shape[1] - 1))
contrasts = {'seed_based_glm': dmn_contrast}

Perform first level analysis#

Setup and fit GLM.

/usr/share/miniconda3/envs/testenv/lib/python3.9/site-packages/nilearn/glm/first_level/first_level.py:64: UserWarning:

Mean values of 0 observed.The data have probably been centered.Scaling might not work as expected

Estimate the contrast.

print('Contrast seed_based_glm computed.')
z_map = first_level_model.compute_contrast(contrasts['seed_based_glm'],
                                           output_type='z_score')

# Saving snapshots of the contrasts
filename = 'dmn_z_map.png'
display = plotting.plot_stat_map(z_map, threshold=3.0, title='Seed based GLM',
                                 cut_coords=pcc_coords)
display.add_markers(marker_coords=[pcc_coords], marker_color='g',
                    marker_size=300)
display.savefig(filename)
print("Save z-map in '{0}'.".format(filename))
plot adhd dmn
Contrast seed_based_glm computed.
Save z-map in 'dmn_z_map.png'.

Generating a report#

It can be useful to quickly generate a portable, ready-to-view report with most of the pertinent information. This is easy to do if you have a fitted model and the list of contrasts, which we do here.

from nilearn.reporting import make_glm_report

report = make_glm_report(first_level_model,
                         contrasts=contrasts,
                         title='ADHD DMN Report',
                         cluster_threshold=15,
                         min_distance=8.,
                         plot_type='glass',
                         )

We have several ways to access the report:

# report  # This report can be viewed in a notebook
# report.save_as_html('report.html')
# report.open_in_browser()

Total running time of the script: ( 0 minutes 18.875 seconds)

Estimated memory usage: 588 MB

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