Comparing connectomes on different reference atlases

This examples shows how to turn a parcellation into connectome for visualization. This requires choosing centers for each parcel or network, via find_parcellation_cut_coords for parcellation based on labels and find_probabilistic_atlas_cut_coords for parcellation based on probabilistic values.

In the intermediary steps, we make use of MultiNiftiLabelsMasker and MultiNiftiMapsMasker to extract time series from nifti objects from multiple subjects using different parcellation atlases.

The time series of all subjects of the brain development dataset are concatenated and given directly to ConnectivityMeasure for computing parcel-wise correlation matrices for each atlas across all subjects.

Mean correlation matrix is displayed on glass brain on extracted coordinates.

Load atlases

from nilearn.datasets import fetch_atlas_yeo_2011, fetch_development_fmri

yeo = fetch_atlas_yeo_2011(n_networks=17)
print(
    "Yeo atlas nifti image (3D) with 17 parcels and liberal mask "
    f" is located at: {yeo['maps']}"
)

[fetch_atlas_yeo_2011] Dataset found in /home/runner/nilearn_data/yeo_2011
Yeo atlas nifti image (3D) with 17 parcels and liberal mask  is located at: /home/runner/nilearn_data/yeo_2011/Yeo_JNeurophysiol11_MNI152/Yeo2011_17Networks_MNI152_FreeSurferConformed1mm_LiberalMask.nii.gz

Load functional data

data = fetch_development_fmri(n_subjects=10)

print(
    "Functional nifti images (4D, e.g., one subject) "
    f"are located at : {data.func[0]!r}"
)
print(
    "Counfound csv files (of same subject) are located "
    f"at : {data['confounds'][0]!r}"
)

[fetch_development_fmri] Dataset found in
/home/runner/nilearn_data/development_fmri
[fetch_development_fmri] Dataset found in
/home/runner/nilearn_data/development_fmri/development_fmri
[fetch_development_fmri] Dataset found in
/home/runner/nilearn_data/development_fmri/development_fmri
Functional nifti images (4D, e.g., one subject) are located at : '/home/runner/nilearn_data/development_fmri/development_fmri/sub-pixar123_task-pixar_space-MNI152NLin2009cAsym_desc-preproc_bold.nii.gz'
Counfound csv files (of same subject) are located at : '/home/runner/nilearn_data/development_fmri/development_fmri/sub-pixar123_task-pixar_desc-reducedConfounds_regressors.tsv'

Extract coordinates on Yeo atlas - parcellations

from nilearn.connectome import ConnectivityMeasure
from nilearn.maskers import MultiNiftiLabelsMasker

# ConnectivityMeasure from Nilearn uses simple 'correlation' to compute
# connectivity matrices for all subjects in a list
connectome_measure = ConnectivityMeasure(kind="correlation", verbose=1)

# create masker using MultiNiftiLabelsMasker to extract functional data within
# atlas parcels from multiple subjects using parallelization to speed up the
# computation
masker = MultiNiftiLabelsMasker(
    labels_img=yeo["maps"],  # Both hemispheres
    standardize_confounds=True,
    memory="nilearn_cache",
    n_jobs=2,
    verbose=1,
)

# extract time series from all subjects
time_series = masker.fit_transform(data.func, confounds=data.confounds)

# calculate correlation matrices across subjects and display
correlation_matrices = connectome_measure.fit_transform(time_series)

# Mean correlation matrix across 10 subjects can be grabbed like this,
# using connectome measure object
mean_correlation_matrix = connectome_measure.mean_

# useful for plotting connectivity interactions on glass brain
from nilearn.plotting import (
    find_parcellation_cut_coords,
    plot_connectome,
    show,
)

# grab center coordinates for atlas labels
coordinates = find_parcellation_cut_coords(labels_img=yeo["maps"])

# plot connectome with 80% edge strength in the connectivity
left_connectome = plot_connectome(
    mean_correlation_matrix, coordinates, edge_threshold="80%"
)

show()

\[MultiNiftiLabelsMasker.fit_transform] Loading regions from '/home/runner/nilea
rn_data/yeo_2011/Yeo_JNeurophysiol11_MNI152/Yeo2011_17Networks_MNI152_FreeSurfer
Conformed1mm_LiberalMask.nii.gz'
\[MultiNiftiLabelsMasker.fit_transform] Resampling regions
\[MultiNiftiLabelsMasker.fit_transform] Finished fit
\[ConnectivityMeasure.wrapped] Finished fit

Note

The approach above will extract time series and compute a single connectivity matrix for both hemispheres. However, the connectome is plotted only for the left hemisphere.

If your aim is to compute and plot hemisphere-wise connectivity, you can follow the example below.

First, create a separate atlas image for each hemisphere:

import nibabel as nb
import numpy as np

from nilearn.image import get_data, new_img_like
from nilearn.image.resampling import coord_transform

# load the atlas image first
label_image = nb.load(yeo["maps"])

# extract the affine matrix of the image
labels_affine = label_image.affine

# generate image coordinates using affine
x, y, z = coord_transform(0, 0, 0, np.linalg.inv(labels_affine))

# generate an separate image for the left hemisphere
# left/right split is done along x-axis
left_hemi = get_data(label_image).copy()
left_hemi[: int(x)] = 0
label_image_left = new_img_like(label_image, left_hemi, labels_affine)

# same for the right hemisphere
right_hemi = get_data(label_image).copy()
right_hemi[int(x) :] = 0
label_image_right = new_img_like(label_image, right_hemi, labels_affine)

Then, create a masker object, compute a connectivity matrix and plot the results for each hemisphere:

for hemi, img in zip(
    ["right", "left"], [label_image_right, label_image_left], strict=False
):
    masker = MultiNiftiLabelsMasker(
        labels_img=img,
        standardize_confounds=True,
        verbose=1,
    )

    time_series = masker.fit_transform(data.func, confounds=data.confounds)

    correlation_matrices = connectome_measure.fit_transform(time_series)
    mean_correlation_matrix = connectome_measure.mean_

    coordinates = find_parcellation_cut_coords(
        labels_img=img, label_hemisphere=hemi
    )

    plot_connectome(
        mean_correlation_matrix,
        coordinates,
        edge_threshold="80%",
        title=f"Yeo Atlas 17 thick (func) - {hemi}",
    )

show()

• 
• 

\[MultiNiftiLabelsMasker.fit_transform] Loading regions from
<nibabel.nifti1.Nifti1Image object at 0x7fe02de48b50>
\[MultiNiftiLabelsMasker.fit_transform] Resampling regions
\[MultiNiftiLabelsMasker.fit_transform] Finished fit
\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe0069b5390>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe0069b5120>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe0069b55a0>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02de48dc0>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe008dcc100>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe008dce710>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe008dcfc40>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe008dce470>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02de48f40>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe008dcea70>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[ConnectivityMeasure.wrapped] Finished fit
\[MultiNiftiLabelsMasker.fit_transform] Loading regions from
<nibabel.nifti1.Nifti1Image object at 0x7fe02de492a0>
\[MultiNiftiLabelsMasker.fit_transform] Resampling regions
\[MultiNiftiLabelsMasker.fit_transform] Finished fit
\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02356e800>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02356d060>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02356dea0>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02356f520>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02356e800>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02399d7e0>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02399e0e0>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02399c6d0>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02399e710>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[MultiNiftiLabelsMasker.fit_transform] Loading data from
<nibabel.nifti1.Nifti1Image object at 0x7fe02399e800>
\[MultiNiftiLabelsMasker.fit_transform] Extracting region signals
\[MultiNiftiLabelsMasker.fit_transform] Cleaning extracted signals
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/joblib/_parallel_backends.py:597: FutureWarning:

boolean values for 'standardize' will be deprecated in nilearn 0.15.0.
Use 'zscore_sample' instead of 'True' or use 'None' instead of 'False'.

\[ConnectivityMeasure.wrapped] Finished fit

Plot a directed connectome - asymmetric connectivity measure

In this section, we use the lag-1 correlation as the connectivity measure, which leads to an asymmetric connectivity matrix. The plot_connectome function accepts both symmetric and asymmetric matrices, but plots the latter as a directed graph.

# Define a custom function to compute lag correlation on the time series
def lag_correlation(time_series, lag):
    n_subjects = len(time_series)
    _, n_features = time_series[0].shape
    lag_cor = np.zeros((n_subjects, n_features, n_features))
    for subject, serie in enumerate(time_series):
        for i in range(n_features):
            for j in range(n_features):
                if lag == 0:
                    lag_cor[subject, i, j] = np.corrcoef(
                        serie[:, i], serie[:, j]
                    )[0, 1]
                else:
                    lag_cor[subject, i, j] = np.corrcoef(
                        serie[lag:, i], serie[:-lag, j]
                    )[0, 1]
    return np.mean(lag_cor, axis=0)


# Compute lag-0 and lag-1 correlations and plot associated connectomes
for lag in [0, 1]:
    lag_correlation_matrix = lag_correlation(time_series, lag)
    plot_connectome(
        lag_correlation_matrix,
        coordinates,
        edge_threshold="90%",
        title=f"Lag-{lag} correlation",
    )

• 
• 

/home/runner/work/nilearn/nilearn/examples/03_connectivity/plot_atlas_comparison.py:200: UserWarning:

'adjacency_matrix' is not symmetric.
A directed graph will be plotted.

Load probabilistic atlases - extracting coordinates on brain maps

from nilearn.datasets import fetch_atlas_difumo
from nilearn.plotting import find_probabilistic_atlas_cut_coords

dim = 64
difumo = fetch_atlas_difumo(dimension=dim, resolution_mm=2)

[fetch_atlas_difumo] Dataset found in /home/runner/nilearn_data/difumo_atlases

Iterate over fetched atlases to extract coordinates - probabilistic

from nilearn.maskers import MultiNiftiMapsMasker

# Create masker using MultiNiftiMapsMasker to extract functional data within
# atlas parcels from multiple subjects using parallelization to speed up the
# computation.
masker = MultiNiftiMapsMasker(
    maps_img=difumo.maps,
    standardize_confounds=True,
    memory="nilearn_cache",
    memory_level=1,
    n_jobs=2,
    verbose=1,
)

# extract time series from all subjects
time_series = masker.fit_transform(data.func, confounds=data.confounds)

# calculate correlation matrices across subjects and display
correlation_matrices = connectome_measure.fit_transform(time_series)

# Mean correlation matrix across 10 subjects can be grabbed like this,
# using connectome measure object
mean_correlation_matrix = connectome_measure.mean_

# grab center coordinates for probabilistic atlas
coordinates = find_probabilistic_atlas_cut_coords(maps_img=difumo.maps)

# plot connectome with 85% edge strength in the connectivity
plot_connectome(
    mean_correlation_matrix,
    coordinates,
    edge_threshold="85%",
    title=f"DiFuMo with {dim} dimensions (probabilistic)",
)
show()

\[MultiNiftiMapsMasker.fit_transform] Loading regions from
'/home/runner/nilearn_data/difumo_atlases/64/2mm/maps.nii.gz'
\[MultiNiftiMapsMasker.fit_transform] Resampling regions
________________________________________________________________________________
[Memory] Calling nilearn.image.resampling.resample_img...
resample_img(<nibabel.nifti1.Nifti1Image object at 0x7fe02356c610>, interpolation='linear', target_shape=(50, 59, 50), target_affine=array([[   4.,    0.,    0.,  -96.],
       [   0.,    4.,    0., -132.],
       [   0.,    0.,    4.,  -78.],
       [   0.,    0.,    0.,    1.]]))
/home/runner/work/nilearn/nilearn/.tox/doc/lib/python3.10/site-packages/nilearn/maskers/nifti_maps_masker.py:413: UserWarning:

Persisting input arguments took 1.18s to run.
If this happens often in your code, it can cause performance problems
(results will be correct in all cases).
The reason for this is probably some large input arguments for a wrapped
 function (e.g. large strings).
THIS IS A JOBLIB ISSUE. If you can, kindly provide the joblib's team with an
 example so that they can fix the problem.

_____________________________________________________resample_img - 1.1s, 0.0min
\[MultiNiftiMapsMasker.fit_transform] Finished fit
\[ConnectivityMeasure.wrapped] Finished fit

Estimated memory usage: 2855 MB